sequence determination meaning

[Medicine]
A multistage process that includes the determination of a sequence (protein,carbohydrate,etc.),its fragmentation and analysis,and the interpretation of the resulting sequence information.

Examples

1. complete sequence determination and structure analysis of genome segment s8 of rice gall dwarf virus
2. In the data collection step, definitive identification of variation is obtained by nucleotide sequence determination of gene fragments.
3. After several rounds of sequence determination, using hybridization of fluorescent labeled probes, a sequence signature of ~ 16-20 bp is determined from each bead.
4. Although the Human Genome Project has progressively improved the fidelity of sequence determination, the overbearing complexity of the genome hinders the identification of associated or ultimately causal variants.
5. Following Sanger's initial report of the reagent, the dinitrofluorobenzene method was widely adopted for studying proteins, until it was superseded by other reagents for terminal analysis ( e . g ., dansyl chloride and later aminopeptidases and carboxypeptidases ) and other general methods for sequence determination ( e . g ., Edman degradation ).